Download Advanced Techniques in Biological Electron Microscopy by John H. Luft (auth.), James K. Koehler Ph. D. (eds.) PDF

By John H. Luft (auth.), James K. Koehler Ph. D. (eds.)

The previous decade has visible a notable bring up within the use of electron microscopy as a researm instrument in biology and medication. hence, so much institu­ tions of upper studying now boast a number of electron optical laboratories having quite a few degrees of class. education within the regimen use of elec­ tron optical apparatus and interpretation of effects is not any longer limited to some prestigious facilities. nevertheless, temniques used by researm employees within the ultrastructural area became super diversified and intricate. even though loads of particularly very good volumes of electron microscopic temnique are actually devoted to the fundamental components on hand whim let the beginner to obtain a cheap advent to the sphere, really few books were dedicated to a dialogue of extra advert­ vanced temnical points of the paintings. It was once with this view that the current quantity used to be conceived as a convenient reference for employees already having a few heritage within the box, as a data resource for these wishing to shift efforts into extra promising temniques, or to be used as a sophisticated direction or seminar advisor. material has been mosen relatively at the foundation of pertinence to provide researm actions in organic electron microscopy and emphasis has been given these components whim appear destined to drastically extend in valuable­ ness within the close to future.

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References BAMFORD, C. , BARB, W. , JENKINS, A. , ONYON, P. : The Kinetics of Vinyl Polymerization by Radical Mechanisms. New York: Academic Press 1958. BAWN, C. E. : The Chemistry of High Polymers. London: Butterworth 1948. : A new staining precedure for electron microscopical cytology. J. Ultra- struct. Res. 27, 250-265 (1969). BEVINGTON, J. : Radical Polymerization. New York: Academic Press 1961. BIRBECK, M. S. , MERCER, E. : Applications of an epoxide embedding medium to electron microscopy.

J. Ultrastruct. Res. 2, 200-214 (1958). SAUNDERS, J. , FRISCH, K. : Polyurethanes. Chemistry and Technology. Part 1. Chemistry. New York: Interscience Publishers 1962. SPURLOCK, B. , KATTINE, V. , FREEMAN, J. : Technical modifications in Maraglas embedding. J. Cell BioI. 17, 203-207 (1963). SPURR, A. : A low-viscosity epoxy resin embedding medium for electron microscopy. J. Ultrastruct. Res. 26, 31-43 (1969). Sterzing, P. , SCALETTI, J. , NAPOLITANO, L. : Tissue cholesterol preservation: solubility of cholesterol digitonide in ethanol.

P. W. P. 045 mm at 20°C. , 940 St. , Milwaukee, Wisc. 53233 under their stock number 13, 956--4. 00 for 100 gm. 26 J. H. LUFT No data are available on the toxicity of diepoxy octane, but it should be somewhat less toxic than either diglycidyl ether or vinylcyclohexene dioxide (SZUBINSKA and LUFT, 1973). 5% DMP-30 for 24 hours at 60°C. The viscosity is even lower than that for diglycidyl ether, and all three resins are compared in Table 4. The cutting properties of these resins are no worse (nor much better) than the other epoxy resins.

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